Codex® HiTemp Reverse Transcriptase maintains performance with degraded RNA
Codex® HiTemp Reverse Transcriptase maintains performance with degraded RNA.
The performance of a one-step RT-qPCR mix containing Codex® HiTemp Reverse Transcriptase and wild-type Taq polymerase was benchmarked against a commercially available one-step RT-qPCR mix (Supplier 1) using RNA with varying degrees of degradation as input. Both one-step RT-qPCR mixes performed similarly for intact and minimally degraded RNA samples (RQS values of 9.1 and 7.2, respectively). However, the Codex® HiTemp Reverse Transcriptase containing mix demonstrated improved performance over Supplier 1 when RNA samples with higher degrees of degradation were used. Here, the ΔCq values between intact and highly degraded RNA were as low as 0.54 cycles for the one-step RT-qPCR mix containing Codex® qPCR Reverse Transcriptase, compared to 0.90 cycles for Supplier 1.