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ECO Synthesis Manufacturing Platform

The scalable RNA manufacturing platform driving higher yields, improved quality, and sustainability through enzymatic innovation.

Redefining how therapeutic RNA is made

ECO Synthesis® Manufacturing Platform replaces traditional solid-phase oligonucleotide synthesis (SPOS) with a flexible, enzyme-catalyzed approach built to scale. Powered by deep enzymology and proprietary process development, it supports fully enzymatic or hybrid “enzymatic/chemical” synthesis workflows, from sequential synthesis to ligation-based assembly. Integrated AI/machine learning-enabled fragment and ligase design further enhances ligation workflows, enabling greater quality, higher yield, and faster turnaround — without harsh solvents or long process cycles.

High-quality, scalable and fast turnaround

Sequential Enzymatic Synthesis

Builds full-length RNA strands one base at a time. Offers clean synthesis, chemical modification compatibility, and scalability under mild, aqueous conditions. Integrated in-process analytics provide real-time oversight and control for reproducible results.

Ligation-Based Assembly

Assembles longer duplexed constructs by ligating short fragments — enabling higher yields, reduced impurities, and modular design strategies.

Hybrid Compatibility

Use ECO Synthesis with ligation-based enzymatically or chemically synthesized fragments. Ideal for therapeutic formats needing flexibility in length, modification, or structure.

Platform Integration

ECO Synthesis integrates seamlessly into existing workflows, enabling scalable enzymatic manufacturing for therapeutic RNA programs.

Why RNA developers choose ECO Synthesis

An enzyme-powered platform purpose-built for scalability, purity, and speed — to deliver high-quality RNAi therapeutics.

Built for scalability

Exceeds 10 kg per run with technology transfer to GMP sites.

Superior process purity

Reduces Class IV impurities and downstream purification burden with cleaner fragment inputs.

Faster production cycles

Supports parallel synthesis and high-efficiency ligation for rapid turnaround.

Greener manufacturing

Aqueous workflows eliminate solvents — reducing waste, hazards, and energy demand.

Custom enzyme fit

Tailored ligases optimize reaction speed, substrate loading, and performance at scale.

Unlock Therapeutic Possibilities at Scale

We make complex science scalable with enzyme-powered solutions that deliver real-world therapeutic impact.

See our solutions

RNA manufacturing

Evolve beyond traditional chemistry with a modern and efficient approach that slots seamlessly into your existing process. Our enzyme-enabled RNA manufacturing platform delivers superior quality, higher yield, better manufacturability and faster results — through a greener solution. A future you can believe in, sooner than you thought possible.

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Pharma biocatalysis

Transform complex, resource-intensive processes into precise, efficient and sustainable breakthroughs. Our proprietary enzymes — powered by CodeEvolver® Technology Platform — enable greener processes, faster pathways and world-class quality from grams to tonnes. Unlock new possibilities with pharma biocatalysisand elevate your process with sustainable chemistry designed to optimize API synthesis.

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Molecular biology and diagnostic applications

Codexis redefines enzyme performance for molecular biology and diagnostic applications. Our CodeEvolver® platform enables tailored enzyme solutions with improved fidelity, enhanced selectivity, and increased activity, delivering faster reaction times to power your applications. As your trusted partner for enzyme evolution, we deliver precision-optimized solutions that power performance.

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