RNAi Therapeutics Overview
Addressing Sustainability of Oligonucleotide Synthesis
ECO Synthesis™ technology (Enzyme-Catalyzed Oligonucleotide Synthesis) is a proprietary new synthesis platform that is being developed for manufacturing RNA oligonucleotide therapeutics at scale. By replacing crucial chemical processes with enzymatic methods, ECO Synthesis™ technology is envisioned to address sustainability and scalability challenges of oligonucleotide manufacturing.
Read Whitepaper
ECO Synthesis™ technology is driven by multiple novel enzymes working as a system to synthesize modified nucleic acids efficiently. The core iterative oligo extension cycle leverages a proprietary terminal deoxynucleotidyl transferase (TdT) enzyme engineered to incorporate common 2’-modified nucleotides, including 2’-F, 2’-OMe, and backbone-modified alpha-phosphorothioate nucleotides, critical for therapeutic RNA applications. Deblocking is achieved enzymatically to close the cyclical process.
Introducing the ECO Synthesis Innovation Lab
Advancing Sustainable, Scalable RNA Manufacturing
Codexis is proud to launch the ECO Synthesis Innovation Lab—a cutting-edge facility driving the future of RNA manufacturing. As the cornerstone of our ECO Synthesis Manufacturing Platform, the lab addresses key challenges in nucleic acid production: scalability, sustainability, speed, and quality.
Highlights of the ECO Synthesis Innovation Lab:
- Engineered ligases for high-yield, high-speed siRNA production
- Advanced analytics for precise quality control
- Scalable workflows tailored to your development stage
Shifting the Paradigm for siRNA Synthesis
Our CodeEvolver® technology platform and experience in enzyme manufacturing come together to enable ECO Synthesis™ technology, with a mission to address the manufacturing obstacles of therapeutic oligonucleotides such as small interfering RNA (siRNA) and antisense oligonucleotides (ASO). The technology strives to provide a transformational alternative to phosphoramidite chemistry and the substantial amount of chemical waste produced.
Read Whitepaper