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A comparison of two RNA synthesis strategies: Hybrid Strategy (left) and Fully Enzymatic (right). The Hybrid Strategy involves ligation using ecoRNA™ Ligase, where chemically or enzymatically synthesized RNA fragments are joined together. The diagram shows multiple RNA fragments being connected by a ligase enzyme. The Fully Enzymatic approach, labeled 'Enzymatic siRNA Synthesis,' illustrates a cyclic process where an extension enzyme adds nucleotides (Extend), followed by a deblocking enzyme removing protective groups (Deblock), with aqueous waste produced at each step.

Enzymatic Manufacturing of siRNA Therapeutics

When manufacturing siRNA for therapeutic applications, Codexis delivers high yields and minimizes unwanted byproducts, while being economically sound. We have a suite of enzymatic pathways to synthesize siRNA, so that you can choose an option that best meets your needs.

The ECO Synthesis™ manufacturing platform (Enzyme-Catalyzed Oligonucleotide Synthesis) is an enzymatic, two-step process that streamlines process development while delivering higher purity siRNA through a scalable, efficient pathway. The RNA Ligase Screening and Optimization Services enables a hybrid approach, where engineered dsRNA (double-stranded RNA) ligases are used to join multiple short, single-stranded RNA (ssRNA) fragments together to form the desired dsRNA duplex.

Step into the Future of Manufacturing RNA

Evolve beyond traditional chemistry with top-tier enzymatic siRNA synthesis.

Enzymatic siRNA Manufacturing Services

Codexis provides a wide range of siRNA manufacturing services using proprietary enzymatic technologies to synthesize your nucleic acid therapeutics for preclinical testing. We can customize analytical methods, tailor final deliverable scales, and provide additional custom services to meet your needs. Additionally, Codexis enables technology transfer of our process to your chosen manufacturing site for scaling up and production under GMP.

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RNA Ligase Screening and Optimization Services

Select from an extensive library of engineered ligases to pick the best variant for one-step assembly of short oligonucleotide fragments for your full-length siRNA asset. Optional ligase optimization services provided in as little as a few weeks to ensure you meet your timelines for clinical and commercial manufacturing needs.

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Scalable, Efficient siRNA Manufacturing

Partner with Codexis for your siRNA manufacturing needs. We bring:

The technology to scale efficiently
The partnership to enable exploration
The legacy to power your project

A visual representation of the RNA development and manufacturing process, divided into three key stages: (1) 'Method Development & Analytics' – covering Analytical Method Development and Analytical Method Qualification, represented with laboratory and data analysis icons; (2) 'Manufacturing the RNA Asset' – including Process Development and Manufacturing, symbolized by gears and machinery; (3) 'Ongoing Support' – covering Reference Standard Generation, Stability Data, and CMC Documentation, represented by a book, a balance scale, and a computer with a document icon. The sections flow sequentially, showing a structured approach to RNA production and quality control.

Related Resources

TIDES US 2024 Presentation

Starting from an enzyme with trace activity and preference for the undesired diastereoisomer, 11 rounds of enzyme evolution were performed.

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RNA Manufacturing Services Brochure

Utilize the ECO Synthesis manufacturing platform to enable scalable enzymatic siRNA synthesis.

Ligase Screening & Optimization Services

Leverage Codexis’ RNA ligase screening and optimization services as a “bridge” to introduce enzymatic solutions into your traditional siRNA manufacturing process.

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Why Codexis?

ECO Synthesis™ technology is a proprietary new synthesis platform that is being developed to produce siRNA more efficiently and sustainably. We do this through:

dsRNA Ligase Screening and Optimization Services to Complement Solid State Synthesis
In-house Ligation Capabilities for GLP-grade siRNA
COMING SOON: ECO Synthesis Innovation Lab for Custom Project Development

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We Are Enabling the Scalable and Efficient Manufacturing of Complex Therapeutics

Our enzymatic tools and platforms enable the scalable and sustainable manufacture of small molecule API and siRNA for our customers while reducing costs and time of manufacturing. Contact us today to learn more about how we can scale your capabilities.