skip to Main Content
Technology
Enable the promise of synthetic biology

Our proprietary CodeEvolver® technology platform accelerates biologic discovery through the transformation of a starting enzyme into a novel biocatalyst or therapeutic candidate, tailored for its desired purpose.

Through a streamlined and iterative process involving library design and construction, protein expression, screening, and sequencing, our technology enables targeted high throughput screening of semi-rational libraries. The platform’s bioinformatic tools guide efficient sampling and selection of improved variants throughout the process.

The resulting evolved variants have enhanced properties such as increased activity, specificity, expression in target hosts, and stability under desired conditions.

Biochemistry-Group2-e1603314414616
Evolve smarter and faster to maximize potential
Efficiency2

Efficient, high volume screening

We design semi-rational libraries by employing site-saturation mutagenesis at many chosen positions based on the property to be improved. Screening at this complex and large scale allows us to identify beneficial genetic diversity. Recombination of this diversity, guided by our proprietary machine-learning software tools, reduces subsequent screening needs and allows for rapid progression through iterative rounds of evolution. This approach allows us to navigate more efficiently through sequence space, quickly reaching effective library sizes greater than 1×1014 variants.

Faster

High-speed directed evolution
To progress rapidly through multiple rounds of evolution, the CodeEvolver® technology platform incorporates contributions from individual mutations that are present in multiple combinatorial contexts to efficiently optimize toward an ideal set of attributes. In the same amount of time other technologies meet a ten-fold improvement over the starting enzyme, we can often advance enzyme performance a thousand-fold to a hundred thousand-fold.

Performance 3

Performance in non-native environments
Our spatially segregated libraries allow us to go beyond target specificity and employ screens for a much broader set of properties: thermostability, pH stability, tolerance to organic solvent, serum stability, expression or uptake into different cell types, and enzymatic activity. This ensures that the final evolved enzyme delivers optimal performance for multiple attributes simultaneously.

The building blocks

Our CodeEvolver® technology platform allows for the development of microorganisms and genes for the benefit of others to produce different variants for use in research and for commercial applications in different industries. It is composed of multiple software tools, laboratory protocols, automated workflows, and proprietary materials that streamline directed evolution by refining and seamlessly integrating each step in the process.

MOSAIC®

The MOSAIC® software program applies a machine-learning computational methodology to determine the effect of mutations on proteins.

Sequences and activity data are used to identify mutations that are beneficial, neutral, and deleterious.  The MOSAIC® program leverages next-generation sequencing data to predict interactions between mutations.

MOSAIC® is a registered trademark/service mark of Codexis, Inc. and is used for directed evolution services, namely, the development of microorganisms and genes for the benefit of others to produce chemicals, biochemicals, enzymes, chemical catalysts, and biochemical catalysts for use in scientific research, medical research, and industrial uses pertaining to chemistry, industrial chemicals, pharmaceuticals, medicine; manufacturing services for others in the fields of enzymes, microorganisms, chemicals, biochemicals, chemical catalysts, and biochemical catalysts. It is also used for chemical research services; scientific research services; research, product development, and process development services for others in the fields of chemistry, industrial chemicals, pharmaceuticals, medicine, and biocatalysis.

ProSAR®

The ProSAR® software program uses linear regression to identify beneficial, neutral, and deleterious mutations from a combinatorial library.

The program requires little sequencing and activity data to make effective predictions on the impact of individual mutations.

The ProSAR® software program uses linear regression to identify beneficial, neutral, and deleterious mutations from a combinatorial library.  The program requires little sequencing and activity data to make effective predictions on the impact of individual mutations.

ProSAR® is a registered trademark/service mark of Codexis, Inc. and is used for scientific research services, scientific testing and assessment of enzymes for use in protein engineering for the benefit of others.

StrategistTM

The Strategist™ software program is a machine-learning tool for library design and data analysis.

The program relies on sequences, activity, and structural data to streamline protein engineering library design workflows.  The visualization tools aid the interpretation of large data sets and enable decision-making.

Strategist™ is a trademark of Codexis, Inc. for downloadable or recorded computer software that uses protein sequencing, activity and 3D structural data for protein library design and analysis, which assists in protein engineering, and aids in interpretation of large protein engineering data sets.

APSTM

The APS™ software program facilitates the creation of oligonucleotide sequences that encode mutations onto a target gene.

The designed oligonucleotides are used for the construction of screening libraries to identify improved properties.

APS™ is a trademark of Codexis, Inc. for downloadable or recorded computer software that facilitates the creation of oligonucleotide sequences encoding genetic mutations for use in research and scientific analysis.

AMELITM

The AMELI™ software program utilizes machine-learning algorithms to recommend enzyme variants that act on a target substrate molecule.

Its enzyme screening database is used to train an algorithm and the graphical interface enables users to search and query that database.

AMELI™ is a trademark of Codexis, Inc. for downloadable or recorded computer software for the storage and searching of screening data for enzymes and substrates for use in research and scientific analysis.

Application

We apply our CodeEvolver® technology platform to design enzymes and other proteins for a wide variety of applications: as biocatalysts for API and fine chemical manufacturing; as life sciences tools; and as novel biologic therapeutic candidates.

There are many steps to a healthier future.
Join us on our journey.
Back To Top
Close search
Search