Higher Substrate Loading
A higher substrate loading can directly increase production yield per batch. Our engineered dsRNA ligases have been tested with substrate concentration at 1.5 mM (or 22 g/L).
RNA Synthesis via Ligation for Improved Scalability and
Reduced Manufacturing Cost
During traditional RNA synthesis, each nucleotide added to the growing oligonucleotide compounds the coupling errors, which decreases the yield of longer RNAs. By utilizing a ligation approach, multiple short, single-stranded RNA (ssRNA) fragments can be ligated together to form the desired double-stranded RNA (dsRNA) duplex. RNA synthesis via ligation can create a higher purity and yield, which improves scalability and significantly reduces manufacturing cost.
Services tailored to your oligonucleotide synthesis scale-up needs
Codexis provides a wide range of research and development services to support oligonucleotide synthesis. Contact us for more information about available services for enzymatic RNA synthesis.
Ligase Screening and Protocol Optimization
- Ligation site selection and RNA fragment design
- Ligase screening against hundreds of natural and engineered enzyme variants
- Ligation protocol optimization by design of experiment (DOE)
Enzyme Customization
- Enzyme engineering to enhance ligation reaction efficiency at the target process conditions
- Research-scale enzyme production under appropriate quality systems to support oligo synthesis process assessment
Research Grade RNA Production
- Product impurity profile assessment
- Ligation process development
- Research-scale RNA production
- Scale-up support
Engineered Ligases for RNA Production
With the proprietary CodeEvolver™ technology platform, we developed a suite of high-performance enzymes to enable the ECO Synthesis™ manufacturing platform as well as support phosphoramidite oligo synthesis through the ligation approach. Key features of our engineered ligases are listed below.