Drop-in solution with better ligation
Compatible with leading sample prep kits
CDX Ligase increases both library yield and complexity when used as a drop-in replacement for the ligase provided in three commercially available library prep kits.
CDX Ligase delivers higher ligation efficiency than T4 DNA Ligase at or above 0.1 µM in a high sensitivity assay with 30 ng 160-mer insert and 200 nM NGS adapter.
E.coli genomic DNA library preparations demonstrate library yields with CDX Ligase to be 1.3-fold to 2.7-fold greater than libraries generated with each respective kit-provided ligase.
Without any prior optimization of buffer composition or other protocol variables, CDX Ligase generates higher library complexity (106% - 167%) relative to the kit-provided ligase.