Engineered for high processivity even through GC-rich and GC-poor (AT-rich) regions, fewer total reads are required to achieve a target mean depth of coverage, presented here alongside the KAPA HyperPrep kit across human WGS.
Critical for applications with low sample inputs such as hybrid capture, Codex® HiFi Hot Start DNA Polymerase with GC enhancer gave high yields in pre-capture amplified libraries during a targeted resequencing workflow, tested among KAPA, Equinox Library Amplification Kit, NEBNext Ultra II Q5 Master Mix, and Platinum Super Fi II Master Mix.
With fidelity improvement of 52X relative to wild-type Taq polymerase, amplification during library preparation can be accomplished with greater confidence in results. In addition to KAPA and NEB Q5, the NEB Q5 Hot Start High-Fidelity 2X Master Mix and ThermoFisher Phusion High-Fidelity DNA Polymerase with GC buffer were tested for comparison.
