Biocatalysts at Codexis

Transaminases perform the asymmetric conversion of ketones to chiral amines using isopropylamine as an amine donor.

Codex® ATA Screening Kit

The Codex® ATA Screening Kit contains 24 amine transaminase enzymes for converting ketones into chiral amines. This selected set of engineered enzymes has a broad substrate range, diverse stereoselectivity, improved solvent stability and can operate on a wide range of temperatures and pHs. 100 g of each kit enzyme is available from stock, and larger quantities up to tons are available on demand. The kit contains 24 x 250 mg enzymes and 300mg PLP cofactor.

Ketoreductases perform the asymmetric reduction of carbonyls to alcohols using nicotinamide cofactors as electron donors.

Codex® KRED Screening Kit

The Codex® KRED Screening Kit contains 24 ketoreductase enzymes for converting ketones into chiral alcohols. This selected set of engineered enzymes has a broad substrate range, diverse stereoselectivity, improved solvent stability and can operate on a wide range of temperatures and pHs. 100 g of each kit enzyme is available from stock, and larger quantities up to tons are available on demand. The kit contains 24 x 250 mg enzymes and a KRED reaction buffer mix.

Ene Reductases perform the symmetric reduction of carbon-carbon double bonds in carbonyl conjugated alkene substrates using nicotinamide cofactors as electron donors.

Codex® ERED Screening Kit

The Codex® ERED Screening Kit contains 7 ene reductase enzymes for asymmetric C=C double bond reduction. This selected set of engineered enzymes has a broad substrate range, diverse stereoselectivity, improved solvent stability and can operate on a wide range of temperatures and pHs. 100 g of each kit enzyme is available from stock, and larger quantities up to tons are available on demand. The kit contains 7 x 250 mg EREDs, NADP+ cofactor, and a cofactor regeneration system.

Nitrilases are enzymes capable of hydrolyzing nitriles to carboxylic acids at neutral pH and ambient temperature.

Codex® NIT Screening Kit

The Codex® NIT Screening Kit contains 12 nitrilase enzymes for converting nitriles into carboxylic acids. This selected set of engineered enzymes has a broad substrate range, diverse stereoselectivity, improved solvent stability and can operate on a wide range of temperatures and pHs.100 g of each kit enzyme is available from stock, and larger quantities up to tons are available on demand. The kit contains 12 x 250 mg enzymes and a NIT reaction buffer mix.

MicroCyp® P450 enzymes provide medicinal chemists and development scientists with unique tools to generate new molecules and metabolites. The Elite MicroCyp® enzymes are improved over our Standard MicroCyp® enzymes and have shown to be more active, have increased solvent tolerance and show greater substrate promiscuity. This makes them a valuable option where novel compounds or larger quantities of products are required.

MicroCyp® Screening Kits

The Codex® Standard MicroCyp® Screening Kit contains 23 of our standard enzymes that are capable of producing mammalian-type metabolites and new compounds for lead diversification. Our MCYP™ enzymes are known to have up to 100-fold increased productivity when compared to Human CYP activity and hence, can be more cost effective when scaling reactions to produce gram quantities of your desired compound. 100 grams of each kit enzyme is available from stock for rapid delivery to our customers for scale-up work. The standard kit contains 23 enzymes arrayed in a 24-well plate, 1 screen per kit and 6 µM of P450 per well. Request a quote using the form below.

Our Elite MicroCyp® enzymes are superior to our Standard MicroCyp® enzymes as they are more active, have increased solvent tolerance and show greater substrate promiscuity, which makes them a valuable option especially where novel compounds or larger quantities are required. The elite kit contains: 11 enzymes arrayed in duplicate wells in a 24-well plate, 2 screens per kit, and 6 µM of P450 per well. Request a quote using the form below.

Imine Reductases perform the asymmetric synthesis of secondary and tertiary chiral amines from imines, or ketones and amines.

Monoamine Oxidases convert amines to imines (asymmetric or resolution) using ambient oxygen as the oxidant.

Our Esterases are capable of amide synthesis using corresponding ester and amine substrates, as well as ester hydrolysis.

Acylases are capable of ester formation/transesterification or ester hydrolysis.

Codexis’ engineered Baeyer-Villiger Monooxygenases perform the asymmetric oxidation of sulfide to sulfoxide using ambient oxygen as the oxidant, and with nicotinamide as a cofactor.

Halohydrin Dehalogenases perform nucleophilic displacement of halogen in a halohydrin.

Cofactors available
NADH, NAD, NADP, NADPH