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Home / Technical Content / Smarter siRNA Ligation: Machine Learning Delivers Precision and Efficiency

Application Note

Smarter siRNA Ligation: Machine Learning Delivers Precision and Efficiency

Ligation-based manufacturing offers a scalable path forward for RNA therapeutics, overcoming the limitations of traditional SPOS as programs expand to larger indications.

This application note reviews how ligation-based methods combined with strategic machine learning can deliver for siRNA manufacturing. The ECO Synthesis® Manufacturing Platform supports both fully enzymatic and hybrid ligation workflows, while integrated ML-enabled fragment and ligase design enhances ligation performance and process predictability.  This combination supports:

  • De-risk ligase selection and fragment design
  • Greater than 80% ligation efficiency guaranteed for a fragment disconnect strategy of your choice
  • Faster turnaround time with reduce the need for lengthy screenings, saving weeks

See how in this application note today. 

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