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Ligation for Improved RNA Manufacturing
AI/Machine Learning-enabled ligation fragments with bespoke ligases deliver scalable and efficient RNA manufacturing by ligation.

Ligation Built for RNA Scalability
Our RNA ligase screening and optimization services enable scalable solutions for complex, high-quality RNA constructs. From fragment design to ligase refinement, we deliver what matters most — substrate loading, yield, and time-to-reaction.
Whether screening your sequence against our expanding ligase library or engineering bespoke variants for tough-to-solve designs, Codexis provides measurable, scalable answers with manufacturability built in.
Ligation-Based RNA Assembly
Codexis uses enzymatic ligation to join short ssRNA fragments into full dsRNA duplexes. This modular approach supports higher yields, greater purity, and scalable assembly — while reducing purification steps and cost. With engineered dsRNA ligases optimized for performance, ligation delivers a robust alternative to traditional coupling chemistry for complex or modified RNA constructs.

The Power of Ligation
Demonstrated advantages in real-world RNAi synthesis
Higher Yield and Purity
Delivers >75% yield and >90% purity while reducing chromatography steps.
Streamlined and Cost-Effective
Process simplification lowers cost of goods and accelerates development timelines.
Scalable and Sustainable
High oligo loading, robust conditions, and greener chemistry enable manufacturing at scale.
Reliable and Compatible
Engineered ligases reduce batch failure and complement solid-phase oligonucleotide synthesis.
Ligase Screening and Optimization Services
Wild-type ligases often fail on challenging substrates. Codexis brings a growing library of engineered ligases or can provide custom enzyme development — enhanced by AI/ML — to shorten optimization cycles and deliver reproducible ligation performance at scale:
- Faster, data-driven process development
- High-yield, efficient manufacturing workflows
- Scalable solutions from early design to GMP readiness
- Enhanced manufacturability and robust performance under real-world conditions
- Complements solid-phase oligonucleotide synthesis for greater flexibility

Platforms built for technical edge
Our proprietary platforms are precision-built to scale where traditional chemistry can’t.
CodeEvolver® Technology Platform
The engine powering scalable solutions others can’t replicate — powered by AI, machine learning, and deep enzymology expertise.
ECO Synthesis® Manufacturing Platform
A modern, scalable RNA manufacturing platform that delivers quality, scalability, and sustainability.
Explore RNA Therapeutic Innovation
Access case studies and whitepapers to see how Codexis is redefining what’s possible in RNA synthesis.






Start Your Ligation Screening Journey
Let’s identify the ligation-based approach that meets your needs today.
