Ligation for Improved RNA Manufacturing

AI/Machine Learning-enabled ligation fragments with bespoke ligases deliver scalable and efficient RNA manufacturing by ligation.

Ligation Built for RNA Scalability

Our RNA ligase screening and optimization services enable scalable solutions for complex, high-quality RNA constructs. From fragment design to ligase refinement, we deliver what matters most — substrate loading, yield, and time-to-reaction.

Whether screening your sequence against our expanding ligase library or engineering bespoke variants for tough-to-solve designs, Codexis provides measurable, scalable answers with manufacturability built in.

Ligation-Based RNA Assembly

Codexis uses enzymatic ligation to join short ssRNA fragments into full dsRNA duplexes. This modular approach supports higher yields, greater purity, and scalable assembly — while reducing purification steps and cost. With engineered dsRNA ligases optimized for performance, ligation delivers a robust alternative to traditional coupling chemistry for complex or modified RNA constructs.

The Power of Ligation

Demonstrated advantages in real-world RNAi synthesis

Higher Yield and Purity

Delivers >75% yield and >90% purity while reducing chromatography steps.

Streamlined and Cost-Effective

Process simplification lowers cost of goods and accelerates development timelines.

Scalable and Sustainable

High oligo loading, robust conditions, and greener chemistry enable manufacturing at scale.

Reliable and Compatible

Engineered ligases reduce batch failure and complement solid-phase oligonucleotide synthesis.

Ligase Screening and Optimization Services

Wild-type ligases often fail on challenging substrates. Codexis brings a growing library of engineered ligases or can provide custom enzyme development — enhanced by AI/ML — to shorten optimization cycles and deliver reproducible ligation performance at scale: