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Sequential Enzymatic RNA Synthesis That Scales
A scalable approach delivering superior yield, quality and sustainability.
The ECO Synthesis® Manufacturing Platform
The ECO Synthesis Manufacturing Platform offers a first-of-its-kind enzymatic alternative to solid-phase oligonucleotide synthesis (SPOS) — delivering speed, scalability, and sustainability for next-gen RNA, including therapeutic siRNA.
It supports two complementary approaches: sequential enzymatic synthesis, which builds RNA strands one nucleotide at a time.
With modular workflows and clean reactions, ECO Synthesis helps you meet aggressive timelines and quality targets while reducing solvent use, waste, and rework. Designed for superior performance across long and modified constructs.
Sequential Enzymatic Synthesis in Action
The ECO Synthesis® Manufacturing Platform applies a repetitive two-step cycle of extension and deblocking to build RNA strands. Each cycle adds a nucleotide under mild, aqueous conditions, then removes the protecting group to prepare for the next addition. This controlled approach supports full-length RNA construction, accommodates chemical modifications, and uses in-process analytics to ensure reproducibility, quality, and scalability.
Two-Step Process
Extension and deblocking cycles create precise, reproducible RNA strands
Mild Conditions
Enzymatic synthesis under aqueous conditions reduces waste and complexity
Scalable Design
Supports long and modified RNA constructs with consistent quality
A Platform Designed for Scalability
ECO Synthesis delivers measurable advantages at every stage.
High Coupling Efficiency
99% average coupling efficiency demonstrated across enzymatic sequential synthesis cycles
Modified Nucleotide Compatibility
Supports 2′-OMe, 2′-F, and PS chemistries under mild aqueous conditions
High Yield & Compatibility
Delivers >75% yield, >90% purity
Streamlined Purification
Fewer process steps and reduced impurities simplify downstream processing and shorten development timelines
Built for the Future of RNAi
ECO Synthesis isn’t just greener — it’s smarter. Our enzyme-powered workflows are engineered to scale with your science, from development to commercial readiness. With superior purity, sustainability, and yield, we’re transforming what’s possible in oligo manufacturing.
Platforms built for technical edge
Our proprietary platforms are precision-built to scale where traditional chemistry can’t.
ECO Synthesis® Manufacturing Platform
A modern, scalable RNA manufacturing platform that delivers quality, scalability, and sustainability.
CodeEvolver® Technology Platform
The engine powering scalable solutions others can’t replicate — powered by AI, machine learning, and deep enzymology expertise.
See ECO Synthesis in action
Explore data-backed performance across purity, timelines, and sustainability.
Unlocking the Future of RNA Manufacturing: A Guide to Enzymatic Synthesis
Machine learning-guided ligation fragment design for efficient siRNA synthesis
Comparative analysis of diastereomeric distribution in siRNA synthesis: SPOS vs enzymatic synthesis
Oligonucleotide CMC for sustainable and scalable enzymatic siRNA manufacturing
Process Development for Enzymatic Synthesis of RNAi Therapeutics
Improving siRNA Production Efficiency
Let’s Build Your RNA Process
Get in touch to discuss synthesis workflows that scale your RNA therapeutic.