Codexis offers Human Cytochrome Biocatalysts,Close
Biocatalyst or Biological CatalystA catalyst derived from a living organism. Enzymes and fermentation strains are common biocatalysts. Biocatalysts significantly speed chemical reactions. the first fully formulated human P450 enzyme CloseEnzymeProteins that act as catalysts, or biocatalysts, in living organisms.system for use in screening and for synthesizing drug metabolites, and MicroCyps, the first commercial source of modified bacterial cytochrome P450 enzymes for producing drug metabolites and generating lead compounds. All of the major human liver cytochromes are available as Human Cytochrome Biocatalysts: 1A2, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4, 3A5, as well as 1A1 (human P450 enzyme predominantly present in the lung). Human Cytochrome Biocatalysts are offered as lyophilized preparations fully formulated with all the necessary components for activity, including a reaction buffer containing a nicotinamide cofactor recycling system. To use, simply add water and the drug to be metabolized. The major applications are for screening to identify the human cytochrome most active in metabolizing a given drug, and scale-up for the production of synthetically useful quantities of drug metabolites. For larger amounts of hydroxylated compounds, MicroCyps are the application of choice since they are engineered to mimic the human enzymes but are more stable than their mammalian counterparts and less sensitive to substrate CloseSubstrateThe biological molecule that an enzyme will act upon. Catalysis occurs when the substrate is broken down, combined with another molecule or changed in some way.and product inhibition.
Codexis also offers metabolite screening and synthesis services, enabling the rapid identification of the human cytochrome(s) involved in the metabolism of a specific drug and the production of the desired metabolites through optimization and scale up. Metabolites can be produced and isolated at the milligram to gram scale for structural identification, toxicity testing, and for use as analytical standards.