Codex™ Biocatalyst Panels have been applied to a number of commercially-relevant projects. Below is an example of a program using the Codex™ KRED Panel. A large-scale screening effort at a pharmaceutical company showed no existing ketoreductase with desired activity and screening of natural enzymes at Codexis similarly did not reveal the desired activity. However, screening of the Codex™ KRED Panel identified multiple variants that had a range of enantioselectivities and activities. Based on the sequence-activity data from the pre-tuned panel, a new plate of variants was designed and customized for this particular chemical process. Many of the resulting fine-tuned biocatalysts showed marked increase in enantioselectivity and activity.
Codex™ Biocatalyst Panels identify biocatalysts with high enantioselectivity and activity: Circles denote the best variant from the Pre-tuned Panel used as a control for the Fine-tuned Panel. The Fine-tuned panel was screened under more demanding conditions to easily identify improved variants. Arrow denotes an improved variant showing exquisite enantioselectivity that nearly converts all of the substrate