Codex™ Biocatalyst Panels have been applied to a number of commercially relevant projects. Below is an example of a program using the Codex™ KRED Panel. A large-scale screening effort at a pharmaceutical company showed no existing ketoreductase with desired activity; screening of natural enzymes at Codexis similarly did not reveal the desired activity. However, screening of the Codex™ KRED Panel identified multiple variants that had a range of enantioselectivities and activities.
Based on the sequence-activity data from the pre-tuned panel, a new plate of variants was designed and customized for this particular chemical process. Many of the resulting biocatalysts showed marked increase in enantioselectivity and activity.
Codex™ Biocatalyst Panels identify biocatalysts with high enantioselectivity and activity: Circles denote the best variant from the pre-tuned panel used as a control for the fine-tuned panel. The fine-tuned panel was screened under more demanding conditions to easily identify improved variants. Arrow denotes an improved variant showing exquisite enantioselectivity that converts nearly all of the substrate to the desired product.